Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
hth

Cell type

Cell type Class
Larvae
Cell type
Imaginal leg disc
NA
NA

Attributes by original data submitter

Sample

source_name
T1 leg imaginal discs, wandering larvae
genotype
isogenic w1118 (BL5905)
chip antibody
Goat anti Hth dG20 (sc-26187, lot A1204)
genome build
dm3

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Tissues were crosslinked with 1% formaldehyde for 10 minutes at RT, and were sonicated. DNA was purified from input and ChIP samples. ChIP-seq libraries were constructed using illumina TruSeq ChIP Library Preparation Kit

Sequencing Platform

instrument_model
NextSeq 500

dm6

Number of total reads
26419843
Reads aligned (%)
95.4
Duplicates removed (%)
43.3
Number of peaks
9365 (qval < 1E-05)

dm3

Number of total reads
26419843
Reads aligned (%)
96.6
Duplicates removed (%)
39.0
Number of peaks
12429 (qval < 1E-05)

Base call quality data from DBCLS SRA