Antigen

Antigen Class

TFs and others

Antigen

hth

Cell type

Cell type Class

Larvae

Cell type

Imaginal leg disc

NA

NA

Sample

source_name

T1 leg imaginal discs, wandering larvae

genotype

isogenic w1118 (BL5905)

chip antibody

Goat anti Hth dG20 (sc-26187, lot A1204)

genome build

dm3

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Tissues were crosslinked with 1% formaldehyde for 10 minutes at RT, and were sonicated. DNA was purified from input and ChIP samples. ChIP-seq libraries were constructed using illumina TruSeq ChIP Library Preparation Kit

Sequencing Platform

instrument_model

NextSeq 500

dm6

Number of total reads

26419843

Reads aligned (%)

95.4

Duplicates removed (%)

43.3

Number of peaks

9365 (qval < 1E-05)

dm3

Number of total reads

26419843

Reads aligned (%)

96.6

Duplicates removed (%)

39.0

Number of peaks

12429 (qval < 1E-05)